St. Louis encephalitis virus (SLE), a member of the flavivirus family, was the most important mosquito-borne arbovirus in North America up until 1999, when West Nile virus (WNV) was introduced into the United States. Wild birds are the maintenance and amplifying hosts of SLE, which is transmitted among birds and to humans by primarily Culex mosquitoes. Human infection with SLE can result in mild to severe illness, with case-fatality rates ranging from 3%-30%.